Putt-Putt delivers a bag of Zoo Chow to the Cartown Zoo for its grand opening. Upon arriving and talking to Outback Al, the head zookeeper, he discovers that many last-minute problems have come up; most notably, several baby animals are missing! Putt-Putt volunteers to travel around the zoo and find Baby Jambo the elephant, Masai the giraffe, Kenya the lion cub, Sammy the seal, Zanzibar the hippopotamus, Skeeter the snake, and Sammy the seal and get them back to their parents.